Calca^{tm2.1(cre/EGFP)Rpa}

The targeting construct was made by inserting a Mt1 gene intron within the open reading frame of mnCre:EGFP, which has a Myc tag (m), followed by a nuclear localization signal (NLS, n), followed by Cre recombinase fused to green fluorescent protein (Cre:GFP), to generate a gene with two exons. Then the second exon was inverted and flanked by a pair of dissimilar frt sites to make a DIO (double-frt inverse open reading frame) construct. Frt-DIO-mnCre:GFP cassette was inserted at the initiation ATG codon in exon 2 of the gene. FLP recombinase inverts the second exon allowing splicing to generate functional Cre recombinase. Frt flanked SV-Neo was removed by crossing with FLPer mice. (J:269999)