An frt-lacZ-loxP-neomycin cassette-frt-loxP construct was inserted into intron 3 and a single loxP site was inserted into intron 4. FLP-mediated recombination removed the lacZ and neomycin cassette, leaving loxP sites flanking exon 4. (J:253972)
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An frt-lacZ-loxP-neomycin cassette-frt-loxP construct was inserted into intron 3 and a single loxP site was inserted into intron 4. FLP-mediated recombination removed the lacZ and neomycin cassette, leaving loxP sites flanking exon 4. (J:253972)