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A 2.64kb eGFP cassette (eGFP-LoxP-FRT-Neo-FRT-LoxP) replaced 5.72Kb of genomic sequence, which flanks the Rtbdn start (ATG) codon and stop (TAG) codon. The neomycin selection cassette was removed via FLP-mediated recombination in the germline. Western blot analysis using an anti-Rtbdn antibody confirmed the absence of RTBDN protein expression in neural retina lysates from homozygous mutant mice. eEGFP expression was found exclusively in the neural retina and absent from all other tissues tested. Immunofluorescence revealed no co-localization of cone arrestin with native eGFP, suggesting that the Rtbdn promoter is not active in cones and it is specific to rod photoreceptors. (J:257162)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
