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CRISPR/Cas9 mutagenesis induced a deletion of 334 base pairs, including the ATG codon. RT-PCR analysis showed that mRNA expression is significantly reduced in homozygous mutant testes. As the primers used for RT-PCR anneal downstream of the deleted region, it appears that mRNA is not transcribed or degraded rapidly. Although decreased protein expression could not be confirmed due to lack of appropriate antibody, authors believe that no protein is produced. (J:253644)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
