Iftap^{tm1b(KOMP)Wtsi}

The L1L2_gt0 cassette was inserted at position 101413619 of Chromosome 2 upstream of critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 5 at position 101414384. Exon 5 is thus flanked by loxP sites. A reporter knockout allele was created by cre recombinase expression in mice carrying the parental Iftaptm1a(KOMP)Wtsi allele to remove loxP-flanked exon 5. RT-PCR and Western blot analysis confirmed the absence of full-length transcript and protein expression, respectively, in homozygous mutant testes. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:236765)