The entire coding sequence was flanked by loxP sites and an FRT-flanked neomycin selection caseette. The selection cassette was removed via FLP-mediated recombination in the germline. (J:275203)
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The entire coding sequence was flanked by loxP sites and an FRT-flanked neomycin selection caseette. The selection cassette was removed via FLP-mediated recombination in the germline. (J:275203)