Grem2^tm1Akh

Most of exon 2 was replaced by a pu(delta)TK (fusion of puromycin and truncated thymidine kinase genes)/EM7neo (kanamycin resistance gene under the synthetic bacterial EM7 promoter) selection cassette via homologous recombination. This resulted in the deletion of the entire coding sequence and most of the 3' untranslated region of the gene, leading to complete loss of the encoded protein. Immunofluorescence analysis revealed that, after myocardial infarction, protein expression was robustly induced in peri-infarct cardiomyocytes of wild-type hearts, whereas protein expression was completely absent in homozygous mutant hearts. (J:250034)