Mcidas^em1Sroy

CRISPR/Cas9 technology was used to generate a deletion of 32 bp in exon 2. This allele is predicted to encode a severely C-terminally truncated protein, lacking all of the required functional domains. Sequence analysis of cDNA obtained from tracheal tissue of homozygous mutant mice confirmed the 32 bp deletion. RT-qPCR analysis revealed a severe reduction of transcript levels in cultures of airway cells from homozygous mutant mice. (J:274880)