The entire locis was flanked by the insertion of an frt-flanked PGK-neo cassette and a loxP site upstream of the gene, and a second loxP site downstream. The neomycin selectino cassette was removed via FLP-mediated recombination. (J:224461)
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The entire locis was flanked by the insertion of an frt-flanked PGK-neo cassette and a loxP site upstream of the gene, and a second loxP site downstream. The neomycin selectino cassette was removed via FLP-mediated recombination. (J:224461)