Mmut^tm2.1Mrb

A conditional ready (floxed) allele was created from the KO first allele chimeras generated using a EUCOMM vector from IKMC project 47381. The cassette for the KO first allele is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon. The critical exon 3 is thus flanked by loxP sites. This conditional ready allele was created by removing the FRT-flanked lacZ sequence , loxP site, neomycin cassette under the control of the human beta-actin promoter and SV40 polyA via flp-mediated recombination, leaving exon 3 flanked by loxP sites. For vector details, see Mmuttm1a(EUCOMM)Wtsi. (J:277356)