Exons 5 and 6 were replaced with a neomycin selection cassette. RT-qPCR analysis confirmed the absence of mRNA expression in E9.5 homozygous mutant embryos. (J:228222)
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Exons 5 and 6 were replaced with a neomycin selection cassette. RT-qPCR analysis confirmed the absence of mRNA expression in E9.5 homozygous mutant embryos. (J:228222)