Thyn1^tm1Osb

This KO first allele (reporter-tagged insertion with conditional potential) was generated using EUCOMM vector PRPG00147_X_4_A07. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons. The critical exons 3-7 are thus flanked by loxP sites. For vector details, see Thyn1tm1a(EUCOMM)Hmgu. (J:277335)