Pex5l^tm1.1Sieg

Using vector PRPGS00115_B_D04 from IMPC, the cassette inserted at position 33004820 of Chromosome 3 upstream of the critical exon 7 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 33004060. The critical exon(s) is/are thus flanked by loxP sites. Flp-mediated recombination removed the reporter and selection cassette. (J:240397)