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The miR-15b/16-2 hairpin spans 430 bp. To generate the miR-15b/16-2 KO targeting vector, DNA fragments flanking miRNAs, with a NotI-BamHI fragment (3.4 kb) as the 5 arm and a HindIII-EcoRI fragment (1.3 kb) as the 3 arm, were isolated from a 129/Sv genomic library and subcloned into a pflox vector. Two selectable markers are incorporated into this vector. The neomycin resistance gene (neo) is flanked by loxP sites and located between the two arms. (J:226920)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
