Rr75^tm1.1Tyag

Mouse BAC RP23-336G22 was used as the basis of the targeting vector. A loxP site was inserted downstream of the sequence containing DNase1 hypersensitivity regions HS19 and HS20 (HS19-20). A loxP and frt site flanked neomycin resistance gene cassette was inserted upstream. The floxed regulatory region sequence and neo cassette were deleted through subsequent cre-mediated recombination. The regulatory regions are located downstream of the Pcdhg cluster (and upstream of the neighboring Diaph1 gene on the reverse strand). Mice with this deletion allele show an average reduction of around 50% in expression of Pcdhb cluster genes. The Pcdhb cluster is located upstream of the Pcdhg cluster and this allele is located around 360kb downstream of the distal-most gene in the Pcdhb cluster. (J:277249)