Gfra1^{tm4.1(cre/ERT2)Jmi}

A gene cassette composed of CreERT2 cDNA-BGH poly(A) followed by a floxed angiotensin converting enzyme (ACE)-Cre and Tn5-neo cassette were introduced into the second exon of the Gfra1 gene by gene targeting. This insertion deleted 95 nucleotides containing the 5'-UTR, the initiator Met and the signal sequences. Upon germ line transmission of the targeted allele, floxed ACE-Cre and Tn5-neo were selfexcised by ACE-Cre-mediated recombination in ES-cell-derived sperm. (J:272383)