Chst14^em6(IMPC)Tcp

This allele from project TCPR259 was generated at The Centre for Phenogenomics.It was targeted by CRISPR/Cas9 nickase-mediated single-strand DNA nicks to induce double-strand breaks repaired by NHEJ causing frame-shift mutation(s) in exon 1 of Chst14. The two guides were targeted to the forward strand of Chr 2:118926702:118926721 and the reverse strand of Chr 2:118926686:118926705 (GRCm38). (J:200814)