The targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked and loxP-flanked neomycin selection cassette downstream of exon 5. Cre-mediated recombination removed the neo cassette and exons 2-5. (J:269891)
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The targeting vector was designed to insert a loxP site upstream of exon 2, and a frt-flanked and loxP-flanked neomycin selection cassette downstream of exon 5. Cre-mediated recombination removed the neo cassette and exons 2-5. (J:269891)