The coding region of the gene was flanked by loxP sites. A frt-flanked PGK-Neo cassette was also inserted in the coding region. Flp-mediated recombination removed the neo cassette. (J:275172)
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The coding region of the gene was flanked by loxP sites. A frt-flanked PGK-Neo cassette was also inserted in the coding region. Flp-mediated recombination removed the neo cassette. (J:275172)