Ranbp1^{Gt(S17-6E1)1.1Sor}

The insertion site was mapped to the 98th base of exon 2 of Ranbp1. The beta-geo reporter is almost undetectable due to the atypical insertion of the reporter into the exon rather than intron. The hygromycin cassette was excised via Flp-mediated recombination. Western blotting and immunofluorescence staining confirmed absence of protein and no 44 amino acid residual protein is detected. (J:235630)