This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTAGGTGCACAAATCCCAT and TTATGTTCACCTCAGCAGCC, which resulted in a 383 bp deletion beginning at Chromosome 5 position 34,426,149 bp and ending after 34,426,531 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001144988 (exon 5) and 148 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 263 and early truncation 4 amino acids later. (J:188991)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count