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CRISPR/Cas9 genome editing is used to insert a 3xFLAG tag sequence at the end of the third domain of the gene (after 5'-CTCCAAGCGTGTGGAGTTC). The PAM site (AGG) was also mutated to AGA to avoid cleaving by Cas9. (J:278920)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
|---|
References Literature
Title
PMID
Journal
Year
IF
