A FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette and left exon 3 floxed. (J:253035)
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A FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette and left exon 3 floxed. (J:253035)