Tg(Myh6-Myl2)97Rbns

A ~5.7 kb genomic fragment, containing the Myh6 (myosin, heavy polypeptide 6, cardiac muscle, alpha) regulatory region, was used to drive the cardiac muscle expression of the mouse slow cardiac regulatory myosin light polypeptide 2 cDNA with Myh7 3' UTR sequence. The genomic fragment contains sequence from a BamHI restriction site in the last intron of the neighboring Myh7 (myosin, heavy polypeptide 7, cardiac muscle, beta) gene to the MaeIII site at 1074 in Myh6 exon 3. It contains the following elements: ~100 bp of the last intron plus the entire last exon of Myh7, ~4 kb of intergenic sequence (containing CCAAT and TAATA boxes and other promoter sequences), and the 5' end of the Myh6 gene (comprising the first two (5' UTR) exons and introns, and most of the the non-coding part of exon 3). The Myl2 cDNA was isolated from a BALB/c adult mouse cardiac cDNA library and includes 70 bp poly(A) sequence. It was tagged with the 3' UTR of the Myh7 gene to be able to distinguish transgenic from endogenous transcripts. The transgenic overexpression of the gene was shown not to affect expression of the endogenous gene. (J:34396)