An frt-flanked neomycin selection cassette was inserted upstream of exon 10 and loxP sites were inserted to flank exons 1-12. FLP-mediated recombination removed the neomycin selection cassette, leaving the loxP sites intact. (J:259650)
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An frt-flanked neomycin selection cassette was inserted upstream of exon 10 and loxP sites were inserted to flank exons 1-12. FLP-mediated recombination removed the neomycin selection cassette, leaving the loxP sites intact. (J:259650)