The targeting vector was designed to insert a loxP site upstream of the entire coding region. A second loxP site and a frt site, followed by neomycin selection cassette (neo), a third loxP site, and a green fluorescent protein (GFP) sequence followed by a second frt site, were inserted downstream of the gene. Flp-mediated recombination removed exon 2. (J:251763)
Basic Information
(C57BL/6NTac x 129S6/SvEvTac)F1
Insertion, Intragenic deletion
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count