A loxP site and rox-flanked PGK-Hygromycin/Zeocin cassette were targeted to intron 1. A 2A peptide, tdTomato cDNA, SV40 polyA cassette, FRT-flanked PGK-Neo cassette, loxP, splice acceptor, EGFP cDNA, and rabbit beta-globin poly adenylation cassette were targeted to exon 12 immediately before the stop codon.Dre- and Flp-mediated recombination excised the rox- and FRT-flanked antibiotic resistance cassettes. (J:341391)
Basic Information
(129S6/SvEvTac x C57BL/6NCrl)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count