Del(6Gimap5-Gimap3)1Yout

This allele was created by crossing mice carrying the Gimap5tm1Yout allele with mice carrying the Gimap3tm1.1Yout and Tg(CAG-cre)13Miya alleles. The Gimap5tm1Yout allele carries two loxP sites flanking the neo cassette inserted into, and replacing the first 35 coding nucleotides of, exon 2. The Gimap3tm1.1Yout allele, which is in the opposite genomic orientation, carries a single loxP site in the 3' UTR of the gene (as a consequence of the deletion of the floxed CDS) in the same orientation as the Gimap5tm1Yout loxP sites. The presence of the Tg(CAG-cre)13Miya allele results in cre-mediated recombination between the loxP sites, thus deleting the entire coding region of Gimap5 and the intergenic sequence between the two genes. This creates a single deletion allele representing a Gimap5 Gimap3 double knockout. (J:209926)