CRISPR/Cas9 genome editing is used to insert an internal ribosome entry site (IRES) fused to a Cre recombinase sequence, a viral 2A oligopeptide (T2A) that mediates ribosomal skipping, and a tdTomato variant of red fluorescent protein (RFP) sequence into the 3'UTR of the gene. (J:279524)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6J
Endonuclease-mediated
Insertion
--
1
--
10

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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