Cep164^{tm1c(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 45720772 of Chromosome 9 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 45721754. Flp-mediated recombination removed the FRT-flanked lacZ and neo cassette leaving exon 4 floxed. (J:255142)