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CRISPR/Cas9 genome editing is used to alter embryonic stem (ES) cells from Krastm4Tyj mutant mice. The original Kras mice have a G12D point mutation in exon 2 of the gene and a loxP ite flanked STOP element in intron 1. Guide RNAs targeting the KrasG12D site and codon 13, an ssODN template and the Cas9 nuclease are used to restore codon 12 to wildtype glycine and introduce a p.G13D mutation associated with coloretal cancer. (J:299292)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
