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CRISPR/Cas9 genome editing is used to alter embryonic stem (ES) cells from Krastm4Tyj mutant mice. The original KrasLSL-G12D mice have a G12D point mutation in exon 2 of the gene and a loxP site flanked STOP element in intron 1. Guide RNAs targeting the KrasG12D site, an ssODN template and the Cas9 nuclease are used to introduce a p.G12C mutation (c.34_36delGGTinsTGC), commonly seen in non-small cell lung cancer. (J:299292)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
