The first loxP was inserted into the 5' region upstream of transcription initiation site, and second loxP together with neomycin cassette was inserted into intron 1. The Neo-cassette was removed using FLPeR mice, leaving exons 1 floxed. (J:271100)
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The first loxP was inserted into the 5' region upstream of transcription initiation site, and second loxP together with neomycin cassette was inserted into intron 1. The Neo-cassette was removed using FLPeR mice, leaving exons 1 floxed. (J:271100)