Kiss1^{tm1.1(cre/EGFP)Rpa}

The targeting construct contains a Cre:GFP fusion carrying Myc 3' UTR pA, and an FRT-SvNeo-FRT cassette introduced to the end of the first exon, such that the sequence between the start codon and the end of that exon are deleted. Flp-mediated recombination removed the FRT-flanked neo cassette. The construct removes the nuclear localization signal from cre, uses a non-optimal initiation codon, removes part of the N-terminal sequence of cre, adds a 3' untranslated region from the Myc gene that promotes a short mRNA half-life, and deletes part of exon 1 that includes the signal peptide. (J:270342)