The coding sequence of the human cDNA was fused at the 5' end to Flag epitope tag sequence and inserted between the CAG promoter and poly(A) signal sequences. The resulting transgene construct was micro-injected into pronuclei of C57BL/6 oocytes. Expression was confirmed by qRT-PCR. (J:270069)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
C57BL/6
--
Insertion
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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