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A BAC containing the genomic sequence of the gene was targeted with a reporter cassette (monomeric red fluorescent protein gene and bovine growth hormone gene poly(A) signal sequences) and an FRT site flanked beta lactamase selection cassette. The reporter cassette replaced the coding sequence of the targeted gene. The selection cassette was subsequently removed through flp-mediated recombination, leaving an allele expressing the reporter gene driven by the promoter of the targeted gene. The transgene construct was micro-injected into pronuclei of oocytes. (J:203286)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
