A loxP site was inserted upstream of the promoter region and an F3 site, attB/P site, HygR cassette, F3 site and loxP site were inserted downstream of exon 1. Targeting was done in ES cells carrying Bcl2a1atm1Tac and Bcl2a1dtm1Tac (J:258915)
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A loxP site was inserted upstream of the promoter region and an F3 site, attB/P site, HygR cassette, F3 site and loxP site were inserted downstream of exon 1. Targeting was done in ES cells carrying Bcl2a1atm1Tac and Bcl2a1dtm1Tac (J:258915)