Exon 3 was flanked by a loxP site on one side and a loxP site flanked neomycin resistance gene cassette on the other. The neo cassette was deleted by transiently transfection of an expression vector of Cre recombinase in the ES cells. (J:265930)
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Exon 3 was flanked by a loxP site on one side and a loxP site flanked neomycin resistance gene cassette on the other. The neo cassette was deleted by transiently transfection of an expression vector of Cre recombinase in the ES cells. (J:265930)