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Cytoplasmic injection of Cas9 mRNA and sgRNAs (5'-GAGAAGGGAAAATTTCTGG-3' and 5'-AGGGCAGAGTGATGTTGTC-3') into zygotes resulted in compound heterozygous F1 mice (from F0 founder crosses) and homozygous F2 mice (from F1 crosses). This allele contains a single T insertion. (J:226780)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
