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The transgene comprises a 6-kb mouse genomic DNA fragment containing exons 1-8 of the gamma-aminobutyric acid type A receptor 6 (Gabra6) gene followed by an internal ribosomal entry site (IRES) and the full-length, 3.1-kb mouse Bmi1 polycomb ring finger oncogene (Bmi1) cDNA. Two (of four generated) transgenic lines, designated T3 and T4, overexpressed Bmi1 mRNA, as determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis, and BMI1 protein, as quantified by immunoblot analysis, in the cerebellum at both postnatal days (P) 9 and P15; the protein was localized to the internal granule cell layer by immunohistochemical labeling of the cerebella of nine-day old mice. (J:193730)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
