Igdmr^em2Takas

This differentially methylated region between Dlk1 and Gtl2 was targeted using CRISPR/Cas9 technology with a pair of sgRNAs flanking the 216 bp CpG-rich tandem repeat array in the center. Line C257 showed a 420 bp deletion that includes the entire repeat sequence and was selected for further study. (J:266963)