Col1a1^{tm1(tetO-GFP/RNAi:Stat3)Ern}

RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Stat3 into the Col1a1 locus. KH2 embryonic stem cells (C57BL/6 x 129S4Sv/Jae), carrying the Gt(ROSA)26Sortm1(rtTA*M2)Jae allele, were previously re-targeted to insert an FRT site-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette in the 3' UTR of the Col1a1 locus, thus adapting the ES cell line for RMCE. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting Stat3 embedded in the Mir30 structure. (J:239149)