Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich

This transgene consists of the rat Kcnj8 cDNA with a glutamine to arginine substation at amino acid 53 (Gly343Asp) and a glycine to aspartate substitution at amino acid 343 (Gly343Asp), rendering ATP-insensitivity, under the control of the CX1 promoter which contains the chicken beta-actin promoter followed by the enhanced green fluorescent protein coding region (including a stop codon) flanked by loxP sites. EGFP is ubiquitously expressed unless cre-recombinase is expressed which excises the EGFP and results in expression of the mutant Kcnj8. Lines 1 and 2 are the high expressing lines, with line 1 entered as the representative line. (J:202037)