Ager^{tm2.1(cre/ERT2)Blh}

The targeting vector was designed to insert a cre/ERT2 fusion gene, followed by a polyadenylation signal and a frt-flanked neomycin resistance cassette, into the start codon of Ager gene. The construct was electroporated into ES cells for homologous recombination. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with FLP-deleter mice to remove the neo cassette. It is a null allele. Ager protein is not detected in lungs of the homozygous mice. (J:265836)