A loxP site was inserted upstream on exon 2 and a loxP site and an FRT flanked pgk-neo cassette were inserted in intron 2 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. (J:240871)
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A loxP site was inserted upstream on exon 2 and a loxP site and an FRT flanked pgk-neo cassette were inserted in intron 2 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. (J:240871)