A loxP site was inserted upstream on exon 2 and a loxP site and an FRT flanked pgk-neo cassette were inserted in intron 2 via homologous recombination. Flp mediated recombination removed the neo cassette leaving exon 2 floxed. (J:240871)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count