P2rx7^tm1.2Jde

The targeting vector consists from 5' to 3': a loxP site followed by the 3'-end (1.4-kb) of mouse intron 1; the murine exon 2 replaced by the human P2RX7 cDNA comprising exons 2-13; a reverse oriented selection marker flanked by frt sites which consists of a phosphoglycerate kinase (PGK) promoter driven neomycin resistance gene equipped with a bovine growth hormone (bGH) poly A signal (pA), a second loxP site and a quadruple poly A signal consisting of a bGHpA, a PGK pA, and 2 SV40 pAs. After germ-line transmission of the modified allele via homologous recombination in ES cells, offspring from male chimeras are bred. Te FRT flanked neo cassette was removed by breeding to deleter-flp mice. The resulting mouse with allele P2rx7 was further bred to deleter-cre mice to remove knock-in human P2RX7 cDNA, thus creating null allele. The loss of functional allele was confirmed by Western blot and functional assay (J:244726). (J:244726)