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The targeting vector is designed with a loxP-flanked transcriptional STOP cassette (PGK-puro-SV40 polyA), and an HA-tagged human DNA-damage inducible transcript 3 (DDIT3)-WPRE-BGH-pA cassette. This vector was inserted into the Gt(ROSA)26Sor locus via a FLP recombinase-mediated cassette exchange method. Specifically, the R26FNF3-1F1 (1F1) ES cell line used to generate the mutation was created by targeting Gt(ROSA)26Sor with a FRT- and F3-flanked PGK-neomycin selection cassette by homologous recombination in EC7.1 ES cells. (J:265800)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
