A floxed duplication of the last exon carrying the E1020K mutation was inserted 3' to the endogenous sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the modified exon. (J:265645)
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A floxed duplication of the last exon carrying the E1020K mutation was inserted 3' to the endogenous sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the modified exon. (J:265645)