One loxP into upstream of exon 2, loxP flanked neo cassette into intron 2 were inserted via homologous recombination. Transient expression of cre in ES cells removed neo cassette leaving exon 2 flanked by loxP. (J:264459)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count