CRISPR/Cas9 is used to remove the single coding exon of the gene. Two single guide RNAs (sgRNAs) were employed: CTGTTAATTAGCCTAACAGG in the region upstream of the coding exon, and TCAGGGTAATTCCCGAAGAG in the downstream region. The intervening sequence was excised. (J:101977)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count